Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: (a) MTT assay (Data represent mean values of four technical and three biological replicates, with error bars indicating standard deviation. Statistical analysis was performed using two-way ANOVA with Dunnett’s post-test (ns p > 0.5, *** p < 0.001, **** p < 0.0001). (b) IC 50 values calculated from MTT assay, and ( c) colony formation assay in PANC-1 and MIA-PaCa-2 cells following 24-h treatment with compound 3a .

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: MTT Assay, Standard Deviation, Colony Assay

Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: Fluorescence microscopy images of MIA-PaCa-2 cells after 24-h treatment with 3a , staining of H 2 -DCFDA (green, ROS), MitoTracker (red, mitochondria), and DAPI (blue, nuclei), along with their merged image. Scale bar is 150 μm.

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: Fluorescence, Microscopy, Staining

Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: Effects of 4a-I on (a) cell viability and (b) colony formation in PANC-1 and MIA-PaCa-2 cells following 24-h treatment. Data represents the MTT assay mean values ± standard deviation from four technical and three biological replicates. Two-way ANOVA determined statistical significance with Dunnett’s posthoc test (ns: p > 0.05).

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: MTT Assay, Standard Deviation

Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: Effects of 5a-I on cell viability in PANC-1 and MIA-PaCa-2 cells following 24-h treatment. Data represents the MTT assay mean values ± standard deviation from four technical and three biological replicates. Two-way ANOVA determined statistical significance with Dunnett’s posthoc test (ns: p > 0.05).

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: MTT Assay, Standard Deviation

Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: Effects of 3b on (a) cell viability and (b) colony formation in PANC-1 and MIA-PaCa-2 cells following 24-h treatment. Data represents the MTT assay mean values ± standard deviation from four technical and three biological replicates. Two-way ANOVA determined statistical significance with Dunnett’s posthoc test (ns: p > 0.05).

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: MTT Assay, Standard Deviation

Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: Effects of 4b-I on (a) cell viability and (b) colony formation in PANC-1 and MIA-PaCa-2 cells following 24-h treatment. Data represents the MTT assay mean values ± standard deviation from four technical and three biological replicates. Two-way ANOVA determined statistical significance with Dunnett’s posthoc test (ns: p > 0.05).

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: MTT Assay, Standard Deviation

Journal: ACS Omega

Article Title: Pyrazole–Cyclotriphosphazene Hybrids: Synthesis, Structural Insights, and Cytotoxic Effects against Pancreatic Cancer Cells

doi: 10.1021/acsomega.5c11955

Figure Lengend Snippet: Effects of 5b-I on (a) cell viability and (b) colony formation in PANC-1 and MIA-PaCa-2 cells following 24-h treatment. Data represent the MTT assay mean values ± standard deviation from four technical and three biological replicates. Two-way ANOVA determined statistical significance with Dunnett’s posthoc test (ns: p > 0.05).

Article Snippet: PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) cells were purchased from American Type Culture Collection (ATCC).

Techniques: MTT Assay, Standard Deviation

Journal: Genes to Cells

Article Title: Molecular Mechanism of Caspase‐8–Dependent Interleukin‐18 Activation in Pancreatic Cancer Cells Induced by 5‐Fluorouracil and Nutrient Starvation

doi: 10.1111/gtc.70111

Figure Lengend Snippet: Induction of cleaved IL‐18 by 5‐FU treatment in human pancreatic cancer cell lines. (A) MIA PaCa‐2 and Panc‐1 cells were cultured in low‐nutrient culture medium and treated with 5‐FU at the indicated concentrations for 48 h. Whole‐cell lysates were analyzed by western blotting with anti‐IL‐18 mAbs. β‐Actin was used as a loading control. (B) Culture supernatants from MIA PaCa‐2 cells treated with 5‐FU for 48 h were analyzed by IL‐18 sandwich ELISA; absorbance (450–620 nm) is shown. * p < 0.05.

Article Snippet: Human pancreatic cancer cell lines (MIA PaCa‐2 and Panc‐1), human colorectal cancer cell line (HCT116), and human cervical cancer cell line (HeLa) were purchased from the American Type Culture Collection.

Techniques: Cell Culture, Western Blot, Control, Sandwich ELISA

Journal: Genes to Cells

Article Title: Molecular Mechanism of Caspase‐8–Dependent Interleukin‐18 Activation in Pancreatic Cancer Cells Induced by 5‐Fluorouracil and Nutrient Starvation

doi: 10.1111/gtc.70111

Figure Lengend Snippet: Cleaved IL‐18 induction by 5‐FU in cancer cells other than pancreatic cancer cells. (A) Representative images of HCT116 and HeLa cells treated with 5‐FU for 48 h in low‐nutrient culture medium (×10). 5‐FU was used at 10 μg/mL for HCT116 cells and 50 μg/mL for HeLa cells. Detached HCT116 cells were observed only after 5‐FU treatment. (B) HCT116 cells treated with 5‐FU were collected as attached or detached fractions; all other samples were collected as whole cells. Lysates were analyzed by western blotting with the indicated antibodies. β‐Actin was used as a loading control.

Article Snippet: Human pancreatic cancer cell lines (MIA PaCa‐2 and Panc‐1), human colorectal cancer cell line (HCT116), and human cervical cancer cell line (HeLa) were purchased from the American Type Culture Collection.

Techniques: Western Blot, Control

Journal: Genes to Cells

Article Title: Molecular Mechanism of Caspase‐8–Dependent Interleukin‐18 Activation in Pancreatic Cancer Cells Induced by 5‐Fluorouracil and Nutrient Starvation

doi: 10.1111/gtc.70111

Figure Lengend Snippet: Proposed model of pyroptosis induction in pancreatic cancer cells. Nutrient starvation induces caspase‐8–dependent cell pyroptosis accompanied by IL‐18 and GSDMD cleavage. 5‐FU increases the number of cells undergoing pyroptosis. Increased release of active IL‐18 can ultimately lead to chronic inflammation in the tumor environment.

Article Snippet: Human pancreatic cancer cell lines (MIA PaCa‐2 and Panc‐1), human colorectal cancer cell line (HCT116), and human cervical cancer cell line (HeLa) were purchased from the American Type Culture Collection.

Techniques:

Journal: Materials Today Bio

Article Title: Escape from cell uptake: Drug-Free cancer therapeutics regulated by hydrophobicity and negative charge

doi: 10.1016/j.mtbio.2025.102752

Figure Lengend Snippet: Cytotoxicity and Cell adsorption property of PVA-U varying G.D. (a) IC 50 values of MIAPaCa-2 cells treated with UDCA, PVA-U3, PVA-U15, and PVA-U25 for 24 h incubation at pH 7.4 and pH 6.5 (n = 3). (b) Cell viability of MIAPaCa-2 cells treated with 10 μg mL −1 PVA-U15 at pH 7.4 and pH 6.5 for 0, 4, 12, and 24 h incubation (n = 3). (c) Confocal images of MIAPaCa-2 cells treated with Cell tracker deep red and 10 μg mL −1 PVA-U0-R, PVA-U3-R, PVA-U15-R, and PVA-U25-R at pH 7.4 and pH 6.5 for 12 h incubation. Cell tracker deep red and PVA-Us were shown in green and red color, respectively. Scale bars of images and enlarge images are 100 μm and 50 μm, respectively. Quantification of intracellular fluorescence intensity of MIAPaCa-2 cells treated with PVA-U0 (black), PVA-U3 (light blue), PVA-U15 (blue), and PVA-U25 (dark blue) incubated for 24 h at pH 7.4 (d) and pH 6.5 (e) (n = 11). Quantification of pericellular fluorescence intensity of MIAPaCa-2 cells treated with PVA-U0 (black), PVA-U3 (light blue), PVA-U15 (blue), and PVA-U25 (dark blue) incubated for 4 h at pH 7.4 (f) and pH 6.5 (g) (n = 11). (h) Confocal images of MIAPaCa-2 cells treated with lysotracker and 10 μg mL −1 PVA-U0-R, PVA-U3-R, PVA-U15-R, and PVA-U25-R at pH 6.5 for 4 h incubation. Lysotracker deep red and PVA-Us were shown in green and red color, respectively. Scale bars of images and enlarge images are 50 μm and 10 μm, respectively. Statistical analysis was performed using unpaired two-tailed Student's t -test. Data are presented as mean ± S.D. ( N.S. : no significant difference, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: MIAPaCa-2 cells, HT-29 cells, and A549 cells were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Adsorption, Incubation, Fluorescence, Two Tailed Test

Journal: Materials Today Bio

Article Title: Escape from cell uptake: Drug-Free cancer therapeutics regulated by hydrophobicity and negative charge

doi: 10.1016/j.mtbio.2025.102752

Figure Lengend Snippet: Antitumor efficacy of PVA-U15. (a) Treatment schedule for MIAPaCa-2 tumors. BALB/c nude mice were inoculated with MIAPaCa-2 cells on day 0. The tumor-bearing mice were randomized and treated with UDCA (144 μM), PVA (10 μg mL −1 ) or PVA-U15 (10 μg mL −1 ). 100 μL of each solution was intratumorally injected 5 days per week from day 9 to day 21. (b) Tumor growth in mice treated with PVA (black), UDCA (blue), and PVA-U15 (red). (c) Body weight of mice treated with PVA (black), UDCA (blue), and PVA-U15 (red). Statistical analysis was performed using Tukey test. Data are presented as mean ± S.D. (∗ p < 0.05).

Article Snippet: MIAPaCa-2 cells, HT-29 cells, and A549 cells were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Injection